Isoenzyme Analysis
Isoenyzyme analysis can determine the species identity of your cell line and determine if your cell line has been contaminated by a cell line of a different species.
Example of isoenzyme gel banding patterns used to determine species identity.
Standard Isoenzyme Analysis includes:
Determination of the isoenzyme gel electrophoresis banding pattern for at least three of the following enzymes:
From the banding patterns of the enzymes used, a "process of elimination" is employed to determine the species identity of the cell line.
Determination of the isoenzyme gel electrophoresis banding pattern for at least three of the following enzymes:
- Glucose - 6 - Phosphate Dehydrogenase (G6PD)
- Lactate Dehydrogenase (LD)
- Malate Dehydrogenase (MD)
- Nucleoside Phosphorylase (NP)
- Aspartate Aminotransferase (AST)
- Peptidase B (PepB)
From the banding patterns of the enzymes used, a "process of elimination" is employed to determine the species identity of the cell line.
This service complies with the Food and Drug Administration's Good Laboratory Practice regulations (Part 58 Title 21 CFR).
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