AGL, Inc. Services


Isoenzyme Analysis

Isoenyzyme analysis can determine the species identity of your cell line and determine if your cell line has been contaminated by a cell line of a different species.

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Example of isoenzyme gel banding patterns used to determine species identity.

 

Standard Isoenzyme Analysis includes:

Determination of the isoenzyme gel electrophoresis banding pattern for at least three of the following enzymes:

  • Glucose - 6 - Phosphate Dehydrogenase (G6PD)
  • Lactate Dehydrogenase (LD)
  • Malate Dehydrogenase (MD)
  • Nucleoside Phosphorylase (NP)
  • Aspartate Aminotransferase (AST)
  • Peptidase B (PepB)

     NOTE: Other isoenzyme analyses are available

 

From the banding patterns of the enzymes used, a "process of elimination" is employed to determine the species identity of the cell line.

 

For more information contact us at 1-800-235-5529 or
email: agl@appliedgenetics.com

 

This service complies with the Food and Drug Administration's Good Laboratory Practice regulations
(Part 58 Title 21 CFR)

 

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